{"id":1008,"date":"2018-08-01T20:11:34","date_gmt":"2018-08-01T20:11:34","guid":{"rendered":"https:\/\/wp.uthscsa.edu\/biochemistry\/?page_id=1008"},"modified":"2021-01-12T17:13:45","modified_gmt":"2021-01-12T23:13:45","slug":"technical-resources","status":"publish","type":"page","link":"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/technical-resources\/","title":{"rendered":"Technical Resources"},"content":{"rendered":"<div class=\"wpb-content-wrapper\" id=\"wpb-content-root\"><p>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<strong>Technical Resources &#8211; Sample Preparation<\/strong><\/p>\n<p><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/biomolecular-nmr-core\/technical-resources\/minimal-salts-media\">Preparation of Minimal Salts Media<\/a><br \/>\n<a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/biomolecular-nmr-core\/technical-resources\/growth-procedure-for-minimal-salts-media\/\">Growth Procedure for H<sub>2<\/sub>O Minimal Salts Media<\/a><br \/>\n<a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/biomolecular-nmr-core\/technical-resources\/growth-of-prototrophic-e-coli-strains-in-d2o-minimal-salts-media\">Growth Procedure for D<sub>2<\/sub>O Minimal Salts Media<\/a><br \/>\n<a href=\"http:\/\/nmr.uthscsa.edu\/html\/education\/dialysis.shtml\">Buffer Exchange Procedure for the Preparation of NMR Samples<\/a><br \/>\n<a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/11\/pH_effect.pdf\">Effect of pH on the NMR Spectra<\/a><\/p>\n<p><strong>Technical Resources &#8211; Operating the NMR Spectrometers &amp; Data Acquisition and Processing<\/strong><\/p>\n<p><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/11\/acqu_guide_final.pdf\">Step-by-step procedure for NMR data acquisition<\/a><br \/>\n<a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/biomolecular-nmr-core\/technical-resources\/chemical-shift-referencing-and-temperature-calibration\">Chemical shift referencing and temperature calibration<\/a><br \/>\n<a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/biomolecular-nmr-core\/technical-resources\/processing-hsqc-data\/\">How to process a 2D HSQC spectrum<\/a><br \/>\n<a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/biomolecular-nmr-core\/technical-resources\/measurement-of-15n-t2-relaxation-time\/\">How to measure and analyze\u00a0<sup>15<\/sup>N T2 relaxation times.<\/a><br \/>\n<a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/11\/Sparky_Instruction2.pdf\">Assigning Proteins Using Sparky<\/a><br \/>\n<a href=\"http:\/\/spin.niddk.nih.gov\/NMRPipe\/newdocs\/ref\/\">NMRPipe Reference Page<\/a><\/p>\n<p><strong>Bruker Manuals<\/strong><\/p>\n<p><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/11\/pulse.pdf\">Pulse program manual<\/a><br \/>\n<a href=\"http:\/\/nmr.uthscsa.edu\/html\/education\/procref.pdf\">Processing manual<\/a><br \/>\n<a href=\"http:\/\/nmr.uthscsa.edu\/html\/education\/acqref.pdf\">Acquisition manual<\/a><\/p>\n<p>&nbsp;[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;]<div class=\"panel outline\"><nav aria-label=\"Biomolecular NMR Core sub-navigation\" class=\"collapsible subnav\"><h2 class=\"close\">Biomolecular NMR Core<\/h2><ul id=\"menu-biomolecular-nmr-core\" class=\"subnav leaf\" data-accordion-menu><li id=\"menu-item-2641\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-2641\"><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/\">Overview<\/a><\/li>\n<li id=\"menu-item-2642\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-2642\"><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/technical-resources\/\">Technical Resources<\/a><\/li>\n<li id=\"menu-item-2643\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-2643\"><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/spectrometers\/\">Technology<\/a><\/li>\n<li id=\"menu-item-2644\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-2644\"><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/quick-guide\/\">Quick Guide to Getting Started (NMR)<\/a><\/li>\n<li id=\"menu-item-2652\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-2652\"><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/quick-guide\/fees\/\">Fees<\/a><\/li>\n<\/ul><\/nav><\/div>[\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]Technical Resources &#8211; Sample Preparation Preparation of Minimal Salts Media Growth Procedure for H2O Minimal Salts Media Growth Procedure for D2O Minimal Salts Media Buffer Exchange Procedure for the Preparation of NMR Samples Effect of pH on the NMR Spectra Technical Resources &#8211; Operating the NMR Spectrometers &amp; Data Acquisition and Processing Step-by-step procedure [&hellip;]<\/p>\n","protected":false},"author":161,"featured_media":0,"parent":174,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/child-page.php","meta":{"footnotes":""},"class_list":["post-1008","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Technical Resources - Biochemistry<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/technical-resources\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Technical Resources - Biochemistry\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]Technical Resources &#8211; Sample Preparation Preparation of Minimal Salts Media Growth Procedure for H2O Minimal Salts Media Growth Procedure for D2O Minimal Salts Media Buffer Exchange Procedure for the Preparation of NMR Samples Effect of pH on the NMR Spectra Technical Resources &#8211; Operating the NMR Spectrometers &amp; Data Acquisition and Processing Step-by-step procedure [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/technical-resources\/\" \/>\n<meta property=\"og:site_name\" content=\"Biochemistry\" \/>\n<meta property=\"article:modified_time\" content=\"2021-01-12T23:13:45+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/biomolecular-nmr-core\\\/technical-resources\\\/\",\"url\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/biomolecular-nmr-core\\\/technical-resources\\\/\",\"name\":\"Technical Resources - Biochemistry\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/#website\"},\"datePublished\":\"2018-08-01T20:11:34+00:00\",\"dateModified\":\"2021-01-12T23:13:45+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/biomolecular-nmr-core\\\/technical-resources\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/biomolecular-nmr-core\\\/technical-resources\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/biomolecular-nmr-core\\\/technical-resources\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Core Facilities\",\"item\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Biomolecular NMR Core\",\"item\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/biomolecular-nmr-core\\\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"Technical Resources\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/#website\",\"url\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/\",\"name\":\"Biochemistry\",\"description\":\"Department of Biochemistry and Structural Biology\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Technical Resources - Biochemistry","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/technical-resources\/","og_locale":"en_US","og_type":"article","og_title":"Technical Resources - Biochemistry","og_description":"[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]Technical Resources &#8211; Sample Preparation Preparation of Minimal Salts Media Growth Procedure for H2O Minimal Salts Media Growth Procedure for D2O Minimal Salts Media Buffer Exchange Procedure for the Preparation of NMR Samples Effect of pH on the NMR Spectra Technical Resources &#8211; Operating the NMR Spectrometers &amp; Data Acquisition and Processing Step-by-step procedure [&hellip;]","og_url":"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/technical-resources\/","og_site_name":"Biochemistry","article_modified_time":"2021-01-12T23:13:45+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/technical-resources\/","url":"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/technical-resources\/","name":"Technical Resources - Biochemistry","isPartOf":{"@id":"https:\/\/lsom.uthscsa.edu\/biochemistry\/#website"},"datePublished":"2018-08-01T20:11:34+00:00","dateModified":"2021-01-12T23:13:45+00:00","breadcrumb":{"@id":"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/technical-resources\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/technical-resources\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/technical-resources\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/lsom.uthscsa.edu\/biochemistry\/"},{"@type":"ListItem","position":2,"name":"Core Facilities","item":"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/"},{"@type":"ListItem","position":3,"name":"Biomolecular NMR Core","item":"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/biomolecular-nmr-core\/"},{"@type":"ListItem","position":4,"name":"Technical Resources"}]},{"@type":"WebSite","@id":"https:\/\/lsom.uthscsa.edu\/biochemistry\/#website","url":"https:\/\/lsom.uthscsa.edu\/biochemistry\/","name":"Biochemistry","description":"Department of Biochemistry and Structural Biology","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/lsom.uthscsa.edu\/biochemistry\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-json\/wp\/v2\/pages\/1008","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-json\/wp\/v2\/users\/161"}],"replies":[{"embeddable":true,"href":"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-json\/wp\/v2\/comments?post=1008"}],"version-history":[{"count":0,"href":"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-json\/wp\/v2\/pages\/1008\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-json\/wp\/v2\/pages\/174"}],"wp:attachment":[{"href":"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-json\/wp\/v2\/media?parent=1008"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}