{"id":851,"date":"2018-07-12T15:40:45","date_gmt":"2018-07-12T15:40:45","guid":{"rendered":"https:\/\/wp.uthscsa.edu\/biochemistry\/?page_id=851"},"modified":"2021-01-27T15:06:56","modified_gmt":"2021-01-27T21:06:56","slug":"crystal-screens-available","status":"publish","type":"page","link":"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/xray-core\/technology\/crystal-screens-available\/","title":{"rendered":"Crystal Screens Available"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]The X-ray Crystallography Core Laboratory utilizes 18 commercial screens containing 96 crystallization reagents. In a &#8220;full screen,&#8221; each commercial screen is set up at two temperatures (22\u00b0C and 4\u00b0C) with proteins of interest to identify initial crystallization conditions. X-ray Core Users will typically provide 750 microliters of a 5-20 mg\/ml protein preparation to complete a full screen. It is recommended to avoid phosphate buffer in order to reduce false positives. HEPES, MES, MOPS, PIPES, CHES, Tris, acetate, citrate and bicine are suitable protein buffers for crystallization. NaCl or KCl up to 150 mM concentration is acceptable. Reducing agents DTT, BME, and TCEP are also compatible with most of the crystallization reagents.<\/p>\n<h2>Qiagen\/Nextal<\/h2>\n<ul>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/Core1.pdf\">JCSG Core 1<\/a><\/li>\n<li><a href=\"https:\/\/3reqjj2w37ot2bnym9qbiae1-wpengine.netdna-ssl.com\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/Core2.pdf\">JCSG Core 2<\/a><\/li>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/Core3.pdf\">JCSG Core 3<\/a><\/li>\n<li><a href=\"https:\/\/3reqjj2w37ot2bnym9qbiae1-wpengine.netdna-ssl.com\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/Core4.pdf\">JCSG Core 4<\/a><\/li>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/Anions.pdf\">Anions<\/a><\/li>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/Cations.pdf\">Cations<\/a><\/li>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/Classics.pdf\">Classics<\/a><\/li>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/PACT.pdf\">Classics II<\/a><\/li>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/PACT.pdf\">PACT<\/a><\/li>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/PEGs.pdf\">PEGsI<\/a><\/li>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/PEGs2.pdf\">PEGs2<\/a><\/li>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/pHClear.pdf\">pH Clear I<\/a><\/li>\n<li><a href=\"https:\/\/3reqjj2w37ot2bnym9qbiae1-wpengine.netdna-ssl.com\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/pHClear2.pdf\">pH Clear II<\/a><\/li>\n<\/ul>\n<h2>Rigaku<\/h2>\n<ul>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/RigakuWizardCryos.pdf\">Wizard Cryos<\/a><\/li>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/RigakuPrecipitantSynergy.pdf\">Precipitant Synergy<\/a><\/li>\n<\/ul>\n<h2>Jena Bioscience<\/h2>\n<ul>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/JBScreenCryo.pdf\">JB Screen Cryos<\/a><\/li>\n<\/ul>\n<h2>Molecular Dimensions<\/h2>\n<ul>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/MIDASplus.pdf\">MidasPlus<\/a><\/li>\n<li>Morpheus<\/li>\n<\/ul>\n<h2>Microlytic<\/h2>\n<ul>\n<li><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/MCSG1.pdf\">MCSG 1<\/a><\/li>\n<li><a href=\"https:\/\/3reqjj2w37ot2bnym9qbiae1-wpengine.netdna-ssl.com\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/MCSG2.pdf\"><span class=\"style2\">MCSG 2<\/span><\/a><\/li>\n<li><span class=\"style2\"><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/MCSG3.pdf\">MCSG 3<\/a><\/span><\/li>\n<li><a style=\"background-color: #ffffff\" href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/wp-content\/uploads\/sites\/39\/2018\/07\/MCSG4.pdf\">MCSG 4<\/a><\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;]<div class=\"panel outline\"><nav aria-label=\"X-ray Crystallography Core Laboratory sub-navigation\" class=\"collapsible subnav\"><h2 class=\"close\">X-ray Crystallography Core Laboratory<\/h2><ul id=\"menu-x-ray-crystallography-core-laboratory\" class=\"subnav leaf\" data-accordion-menu><li id=\"menu-item-2615\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-2615\"><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/xray-core\/\">Overview<\/a><\/li>\n<li id=\"menu-item-2617\" class=\"menu-item menu-item-type-custom menu-item-object-custom menu-item-2617\"><a href=\"https:\/\/uthscsa.corefacilities.org\/service_center\/show_external\/3542\">Services<\/a><\/li>\n<li id=\"menu-item-2623\" class=\"menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-2623\"><a href=\"#\">Technology<\/a>\n<ul class=\"sub-menu\">\n\t<li id=\"menu-item-2616\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-2616\"><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/xray-core\/technology\/\">Technology Overview<\/a><\/li>\n\t<li id=\"menu-item-2624\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-2624\"><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/xray-core\/technology\/crystal-screens-available\/\">Crystal Screens Available<\/a><\/li>\n\t<li id=\"menu-item-2626\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-2626\"><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/xray-core\/technology\/beam-center-positions\/\">Beam Center Positions<\/a><\/li>\n<\/ul>\n<\/li>\n<li id=\"menu-item-2618\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-2618\"><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/xray-core\/publications\/\">Publications<\/a><\/li>\n<li id=\"menu-item-2829\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-2829\"><a href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/xray-core\/technology\/lab-guidelines\/\">Lab Guidelines<\/a><\/li>\n<\/ul><\/nav><\/div>[\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]The X-ray Crystallography Core Laboratory utilizes 18 commercial screens containing 96 crystallization reagents. In a &#8220;full screen,&#8221; each commercial screen is set up at two temperatures (22\u00b0C and 4\u00b0C) with proteins of interest to identify initial crystallization conditions. X-ray Core Users will typically provide 750 microliters of a 5-20 mg\/ml protein preparation to complete [&hellip;]<\/p>\n","protected":false},"author":161,"featured_media":0,"parent":503,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/child-page.php","meta":{"footnotes":""},"class_list":["post-851","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.9 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Crystal Screens Available - Biochemistry<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/xray-core\/technology\/crystal-screens-available\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Crystal Screens Available - Biochemistry\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]The X-ray Crystallography Core Laboratory utilizes 18 commercial screens containing 96 crystallization reagents. In a &#8220;full screen,&#8221; each commercial screen is set up at two temperatures (22\u00b0C and 4\u00b0C) with proteins of interest to identify initial crystallization conditions. X-ray Core Users will typically provide 750 microliters of a 5-20 mg\/ml protein preparation to complete [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/xray-core\/technology\/crystal-screens-available\/\" \/>\n<meta property=\"og:site_name\" content=\"Biochemistry\" \/>\n<meta property=\"article:modified_time\" content=\"2021-01-27T21:06:56+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/xray-core\\\/technology\\\/crystal-screens-available\\\/\",\"url\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/xray-core\\\/technology\\\/crystal-screens-available\\\/\",\"name\":\"Crystal Screens Available - Biochemistry\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/#website\"},\"datePublished\":\"2018-07-12T15:40:45+00:00\",\"dateModified\":\"2021-01-27T21:06:56+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/xray-core\\\/technology\\\/crystal-screens-available\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/xray-core\\\/technology\\\/crystal-screens-available\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/xray-core\\\/technology\\\/crystal-screens-available\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Core Facilities\",\"item\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"X-ray Crystallography Core Laboratory\",\"item\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/xray-core\\\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"Technology (X-ray Crystallography Core Laboratory)\",\"item\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/core-facilities\\\/xray-core\\\/technology\\\/\"},{\"@type\":\"ListItem\",\"position\":5,\"name\":\"Crystal Screens Available\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/#website\",\"url\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/\",\"name\":\"Biochemistry\",\"description\":\"Department of Biochemistry and Structural Biology\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/biochemistry\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Crystal Screens Available - Biochemistry","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/lsom.uthscsa.edu\/biochemistry\/core-facilities\/xray-core\/technology\/crystal-screens-available\/","og_locale":"en_US","og_type":"article","og_title":"Crystal Screens Available - Biochemistry","og_description":"[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]The X-ray Crystallography Core Laboratory utilizes 18 commercial screens containing 96 crystallization reagents. 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