{"id":1707,"date":"2023-06-27T16:03:01","date_gmt":"2023-06-27T21:03:01","guid":{"rendered":"https:\/\/lsom.uthscsa.edu\/molecular-medicine\/?p=1707"},"modified":"2023-06-27T16:04:45","modified_gmt":"2023-06-27T21:04:45","slug":"ewsr1-maintains-centromere-identity","status":"publish","type":"post","link":"https:\/\/lsom.uthscsa.edu\/molecular-medicine\/2023\/06\/27\/ewsr1-maintains-centromere-identity\/","title":{"rendered":"EWSR1 Maintains Centromere Identity"},"content":{"rendered":"<p>Abstract<br \/>\nThe centromere is essential for ensuring high-fidelity transmission of chromosomes. CENP-A, the centromeric histone H3 variant, is thought to be the epigenetic mark of centromere identity. CENP-A deposition at the centromere is crucial for proper centromere function and inheritance. Despite its importance, the precise mechanism responsible for maintenance of centromere position remains obscure. Here, we report a mechanism to maintain centromere identity. We demonstrate that CENP-A interacts with EWSR1 (Ewing sarcoma breakpoint region 1) and EWSR1-FLI1 (the oncogenic fusion protein in Ewing sarcoma). EWSR1 is required for maintaining CENP-A at the centromere in interphase cells. EWSR1 and EWSR1-FLI1 bind CENP-A through the SYGQ2 region within the prion-like domain, important for phase separation. EWSR1 binds to R-loops through its RNA-recognition motif in vitro. Both the domain and motif are required for maintaining CENP-A at the centromere. Therefore, we conclude that EWSR1 guards CENP-A in centromeric chromatins by binding to centromeric RNA.<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37243594\/\">Kitagawa R, Niikura Y, Becker A, Houghton PJ,\u00a0Kitagawa K.<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Abstract The centromere is essential for ensuring high-fidelity transmission of chromosomes. CENP-A, the centromeric histone H3 variant, is thought to be the epigenetic mark of centromere identity. CENP-A deposition at the centromere is crucial for proper centromere function and inheritance. Despite its importance, the precise mechanism responsible for maintenance of centromere position remains obscure. Here, [&hellip;]<\/p>\n","protected":false},"author":315,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"class_list":["post-1707","post","type-post","status-publish","format-standard","hentry","category-highimpactpublication"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>EWSR1 Maintains Centromere Identity - Department of Molecular Medicine<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lsom.uthscsa.edu\/molecular-medicine\/2023\/06\/27\/ewsr1-maintains-centromere-identity\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"EWSR1 Maintains Centromere Identity - Department of Molecular Medicine\" \/>\n<meta property=\"og:description\" content=\"Abstract The centromere is essential for ensuring high-fidelity transmission of chromosomes. CENP-A, the centromeric histone H3 variant, is thought to be the epigenetic mark of centromere identity. CENP-A deposition at the centromere is crucial for proper centromere function and inheritance. Despite its importance, the precise mechanism responsible for maintenance of centromere position remains obscure. 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