BCL2::IGH translocation major breakpoint (MBR) by real-time PCR
Hematopathology, MBR, , follicular lymphoma, diffuse large B-cell lymphoma, BCL2::IGH, BCL2::IGH, BCL2/JH, t(14;18)
Molecular Diagnostics Laboratory
Clinical Indication and Relevance
The assay can confirm the initial diagnosis of major-breakpoint (MBR) BCL2::IGH translocation-positive follicular lymphoma or diffuse large B-cell lymphoma. It can also be used to monitor minimal residual disease in MBR-positive patients.
DNA is isolated and amplified with specific primers targeting the BCL2/JH major breakpoint (MBR) using real-time PCR. PCR product is then sized by capillary gel electrophoresis. Results are reported as positive or negative for BCL2 MBR. The assay’s analytic detection limit is 1 tumor cell in 1000 normal cells.
Minimum Specimen Requirements
- Peripheral blood (PB): 3-5 mL, in purple top (sodium EDTA) tube.
- Bone marrow (BM): 1-3 mL, drawn into a syringe containing anticoagulant and then delivered in purple top tube.
- Fresh or frozen tissue: fresh tissue should be obtained in a sterile manner, and a minimum 3 mm3 of tissue is required. Fresh tissue should be placed in culture medium for transport or snap frozen (see transport section below).
- Formalin-fixed, paraffin-embedded (FFPE) tissue blocks: send tissue block to the lab, or contact lab for instructions regarding cutting paraffin sections for molecular studies.
- PB or BM sample, deliver immediately at 2-8°C (wet ice or cold packs). Do not freeze.
- Tissue samples should be delivered at room temperature in RPMI culture medium to the lab within 3 hours of collection, or snap frozen in liquid nitrogen at -70°C and packed in dry ice for delivery. Please do not allow frozen tissues to thaw.
- Formalin-fixed paraffin-embedded tissue blocks can be delivered at room temperature.
Stability PB or BM samples: ambient – 1 hour; refrigerated – 48 hours. Unacceptable Samples
- Serum or plasma; frozen PB or BM; clotted blood; severely hemolyzed samples
- Paraffin tissue fixed in Zenker’s, B5, or Bouin’s fixatives
- Bone marrow biopsy decalcified in formic acid
Five to seven working daysView Requisition Sheet