BCL2 translocation major breakpoint by real-time PCR

CPT Code(s)

81401, G0452-26

Synonym(s)

Hematopathology, MBR

Performed

Molecular Diagnostics Laboratory

Methodology

DNA is isolated and amplified with specific primers targeting the BCL2/JH major breakpoint
(MBR) using real-time PCR. PCR product is then sized by capillary gel electrophoresis. Results
are reported as positive or negative for BCL2 MBR. The assay’s analytic detection limit is 1
tumor cell in 1000 normal cells.

Minimum Specimen Requirements

Collect

  • Peripheral blood (PB): 3-5 mL, in purple top (sodium EDTA) tube.
  • Bone marrow (BM): 1-3 mL, drawn into a syringe containing anticoagulant and then
    delivered in purple top tube.
  • Fresh or frozen tissue: fresh tissue should be obtained in a sterile manner, and a minimum
    3 mm3
    of tissue is required. Fresh tissue should be placed in culture medium for transport
    or snap frozen (see transport section below).
  • Formalin-fixed, paraffin-embedded (FFPE) tissue blocks: send tissue block to the lab, or
    contact lab for instructions regarding cutting paraffin sections for molecular studies.

Transport

  • PB or BM sample, deliver immediately at 2-8°C (wet ice or cold packs). Do not freeze.
  • Tissue samples should be delivered at room temperature in RPMI culture medium to the
    lab within 3 hours of collection, or snap frozen in liquid nitrogen at -70°C and packed in
    dry ice for delivery. Please do not allow frozen tissues to thaw.
  • Formalin-fixed paraffin-embedded tissue blocks can be delivered at room temperature.

Stability
PB or BM samples: ambient – 1 hour; refrigerated – 48 hours.

Unacceptable Samples

  • Serum or plasma; frozen PB or BM; clotted blood; severely hemolyzed samples
  • Paraffin tissue fixed in Zenker’s, B5, or Bouin’s fixatives
  • Bone marrow biopsy decalcified in formic acid

Turnaround time

Five to seven working days