{"id":1213,"date":"2018-12-20T16:28:31","date_gmt":"2018-12-20T16:28:31","guid":{"rendered":"https:\/\/wp.uthscsa.edu\/pathology\/?page_id=1213"},"modified":"2020-04-28T22:09:56","modified_gmt":"2020-04-28T22:09:56","slug":"fish-t-cell-lymphoma","status":"publish","type":"page","link":"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/clinical-molecular-cytogenetics\/fish-t-cell-lymphoma\/","title":{"rendered":"FISH T-cell Lymphoma"},"content":{"rendered":"

[vc_row][vc_column width=”2\/3″][vc_column_text]<\/p>\n

Fluorescence In Situ Hybridization \u2013 T-cell Lymphoma Panel<\/h3>\n

Test Overview<\/p>\n

CPT Code(s)<\/strong><\/p>\n

88237-52, 88271\u00d73, 88275, 88291<\/p>\n

Methodology<\/strong><\/p>\n

Culture\/Hybridization\/Microscopy\/Interpretation<\/p>\n

Specimen Requirements<\/strong><\/p>\n\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t
Bone Marrow and\/or Leukemia Blood<\/th>Lymph Node<\/th>\n<\/tr>\n<\/thead>\n
Container: Sodium Heparin (green top tube)<\/td>Container: Transport Media\/Saline<\/td>\n<\/tr>\n
Optimal Quantity: 2-3 ml<\/td>Optimal Quantity: 23\u00a0cm<\/td>\n<\/tr>\n
Minimum Quantity: 1-2 ml<\/td>Minimum Quantity: 13\u00a0cm<\/td>\n<\/tr>\n
Storage: Room Temperature<\/td>Storage: Room Temperature<\/td>\n<\/tr>\n
Stability at Room Temperature: 8 hours, then refrigerate<\/td>Stability at Room Temperature: 8 hours, then refrigerate<\/td>\n<\/tr>\n
Transportation: Avoid freezing or heating over 35oC<\/td>Transportation: Avoid freezing or heating over 35oC<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n

Turnaround Time<\/strong><\/p>\n

Final report in 5 days for 90% cases<\/p>\n

Test Details<\/p>\n

Clinical Significance<\/strong><\/p>\n

Fluorescence in situ hybridization (FISH) is a sensitive method to detect smaller genomic changes associated with various hematological malignancies and solid tumors.\u00a0\u00a0There are several advantages with FISH technology over routine chromosome analysis and such advantages include the ability of FISH technology to detect genomic abnormalities in non-viable and non-dividing tissues, rapid turnaround time, and increased resolution.\u00a0\u00a0However, FISH technology is complementary to routine chromosome analysis and cannot substitute routine chromosome analysis for diagnosis of cancer.<\/p>\n

Clinical Background<\/strong><\/p>\n

The common genomic abnormalities seen in T-cell lymphoma usually involve the T-cell receptor genes (TCR) with various transcription factors.\u00a0\u00a0The genes that are commonly involved are TCR-alpha (TCRA) and TCR-delta (TCRD) at 14q11, TCR-beta (TCRB) at 7q35, TCR-gamma (TCRG) at 7p15.\u00a0\u00a0The ALK gene rearrangements occur in about 2% of adults and 13% of children.<\/p>\n

Methods<\/strong><\/p>\n

The samples are usually cultured for 24-hours before hybridizing with the probes.\u00a0\u00a0After hybridization and post-hybridization washes, the slides\u00a0are\u00a0analyzed using a fluorescence microscope equipped with\u00a0epifluorescence\u00a0and appropriate filter sets.\u00a0\u00a0Analysis and scoring is carried out by two certified technologists in a blinded fashion.\u00a0\u00a0Results are reviewed by both the laboratory manager and the director.\u00a0\u00a0The T-cell lymphoma FISH panel includes the following probes.<\/p>\n\n\n\n\n\t\n\t\n\t
inv(7) \/ iso(7q)\t<\/td>TCRB\t<\/td>Cytocell (cat # LPH048)
\n<\/td>\n<\/tr>\n
inv(14) \/ 14q rearrangement\t<\/td>TCRAD\t<\/td>Cytocell (cat # LPH047)
\n<\/td>\n<\/tr>\n
t(2;5) \/ 2p rearrangement\t<\/td>ALK\t<\/td>Cytocell (cat # LPS 019)
\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n

\u00a0<\/strong>Interpretation of Results<\/strong><\/p>\n

The morphologic interpretation and correlation of results on all cases is performed by a board-certified doctoral level scientist (laboratory director).\u00a0The final report has a narrative description of results.\u00a0\u00a0If abnormalities are present, they are explained in a paragraph which helps to clarify and correlate chromosomal findings with phenotype.\u00a0References are included in the report to help the referring physician with interpretation, which include books or journals that contain appropriate information. Recommendations are made as to any additional testing, if necessary.[\/vc_column_text][\/vc_column][vc_column width=”1\/3″][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column width=”2\/3″][vc_column_text] Fluorescence In Situ Hybridization \u2013 T-cell Lymphoma Panel Test Overview CPT Code(s) 88237-52, 88271\u00d73, 88275, 88291 Methodology Culture\/Hybridization\/Microscopy\/Interpretation Specimen Requirements Turnaround Time Final report in 5 days for 90% cases Test Details Clinical Significance Fluorescence in situ hybridization (FISH) is a sensitive method to detect smaller genomic changes associated with various hematological malignancies […]<\/p>\n","protected":false},"author":161,"featured_media":0,"parent":615,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/child-page.php","meta":{"footnotes":""},"class_list":["post-1213","page","type-page","status-publish","hentry"],"yoast_head":"\nFISH T-cell Lymphoma - Department of Pathology and Laboratory Medicine<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/clinical-molecular-cytogenetics\/fish-t-cell-lymphoma\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FISH T-cell Lymphoma - 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