{"id":836,"date":"2018-12-11T20:35:34","date_gmt":"2018-12-11T20:35:34","guid":{"rendered":"https:\/\/wp.uthscsa.edu\/pathology\/?page_id=836"},"modified":"2026-02-02T12:04:13","modified_gmt":"2026-02-02T18:04:13","slug":"muscle-enzyme-histochemistry","status":"publish","type":"page","link":"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/histology-immunohistochemistry-laboratory\/laboratory-services-2\/muscle-enzyme-histochemistry\/","title":{"rendered":"Muscle Enzyme Histochemistry"},"content":{"rendered":"

[vc_row][vc_column width=”2\/3″][vc_column_text]Muscle biopsy is an essential component of the investigation of patients with neuromuscular disorders (and is also often employed in research studies on the effects of exercise on muscle). In order to gain maximum information the SPECIMENS for light microscopy and chemical analysis should NOT BE FIXED but prepared for cryostat sectioning in order to allow for morphological, morphometric, enzyme histochemical and immunocytochemical investigations. Material may also be required for electron microscopy, biochemical or DNA studies. Histochemical enzyme technique on cryostat sections of muscle biopsies provide a better opportunity for defining fiber types and detecting any alteration in muscle fibers. Muscle biopsies are useful in diagnosing denervating disease through myofiber type atrophy or groupings; certain dystropic fibers are deficient in type 2B fibers which is also detectable with enzyme histochemistry procedures.<\/p>\n

\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
Method<\/th>Use<\/th>\n<\/tr>\n<\/thead>\n
Hematoxylin and eosin*<\/td>Muscle fibre architecture, nuclear position and number, degeneration and regeneration, hyaline fibres, fibrosis.<\/td>\n<\/tr>\n
Modified Gomori trichrome<\/td>Nemaline rods and \u2018ragged-red\u2019 fibres<\/td>\n<\/tr>\n
Myofibrillar ATPase*<\/td>Types I, IIa, IIb, IIc<\/td>\n<\/tr>\n
(pH 4.3, 4.6, 10.2)<\/td>Type grouping<\/td>\n<\/tr>\n
NADH-Tr*<\/td>Mitochondrial and tubular aggregates<\/td>\n<\/tr>\n
Cytochrome oxidase<\/td>Mitochondria, enzyme deficiency<\/td>\n<\/tr>\n
Phosphorylase*<\/td>Enzyme deficiency<\/td>\n<\/tr>\n
Non-specific esterase*<\/td>Inflammation, lysosomes<\/td>\n<\/tr>\n
PAS*<\/td>Glycogen, carbohydrate<\/td>\n<\/tr>\n
Oil red O*<\/td>Lipid<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][\/vc_column][vc_column width=”1\/3″][vc_column_text css=””]<\/p>\n

Laboratory Services<\/a><\/h3>\n

Specimen Requirements & Testing Schedule<\/a><\/h3>\n

[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column width=”2\/3″][vc_column_text]Muscle biopsy is an essential component of the investigation of patients with neuromuscular disorders (and is also often employed in research studies on the effects of exercise on muscle). In order to gain maximum information the SPECIMENS for light microscopy and chemical analysis should NOT BE FIXED but prepared for cryostat sectioning in order […]<\/p>\n","protected":false},"author":161,"featured_media":0,"parent":825,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/child-page.php","meta":{"footnotes":""},"class_list":["post-836","page","type-page","status-publish","hentry"],"yoast_head":"\nMuscle Enzyme Histochemistry - Department of Pathology and Laboratory Medicine<\/title>\n<meta name=\"description\" content=\"Muscle biopsy is an essential component of the investigation of patients with neuromuscular disorders (and is also often employed in research studies on the effects of exercise on muscle).\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/histology-immunohistochemistry-laboratory\/laboratory-services-2\/muscle-enzyme-histochemistry\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Muscle Enzyme Histochemistry - Department of Pathology and Laboratory Medicine\" \/>\n<meta property=\"og:description\" content=\"Muscle biopsy is an essential component of the investigation of patients with neuromuscular disorders (and is also often employed in research studies on the effects of exercise on muscle).\" \/>\n<meta property=\"og:url\" content=\"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/histology-immunohistochemistry-laboratory\/laboratory-services-2\/muscle-enzyme-histochemistry\/\" \/>\n<meta property=\"og:site_name\" content=\"Department of Pathology and Laboratory Medicine\" \/>\n<meta property=\"article:modified_time\" content=\"2026-02-02T18:04:13+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/pathology\\\/reference-labs\\\/histology-immunohistochemistry-laboratory\\\/laboratory-services-2\\\/muscle-enzyme-histochemistry\\\/\",\"url\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/pathology\\\/reference-labs\\\/histology-immunohistochemistry-laboratory\\\/laboratory-services-2\\\/muscle-enzyme-histochemistry\\\/\",\"name\":\"Muscle Enzyme Histochemistry - Department of Pathology and Laboratory Medicine\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/pathology\\\/#website\"},\"datePublished\":\"2018-12-11T20:35:34+00:00\",\"dateModified\":\"2026-02-02T18:04:13+00:00\",\"description\":\"Muscle biopsy is an essential component of the investigation of patients with neuromuscular disorders (and is also often employed in research studies on the effects of exercise on muscle).\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/pathology\\\/reference-labs\\\/histology-immunohistochemistry-laboratory\\\/laboratory-services-2\\\/muscle-enzyme-histochemistry\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/lsom.uthscsa.edu\\\/pathology\\\/reference-labs\\\/histology-immunohistochemistry-laboratory\\\/laboratory-services-2\\\/muscle-enzyme-histochemistry\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/pathology\\\/reference-labs\\\/histology-immunohistochemistry-laboratory\\\/laboratory-services-2\\\/muscle-enzyme-histochemistry\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/pathology\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Laboratories of Department of Pathology and Laboratory Medicine\",\"item\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/pathology\\\/reference-labs\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Histology and Immunohistochemistry Laboratory\",\"item\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/pathology\\\/reference-labs\\\/histology-immunohistochemistry-laboratory\\\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"Laboratory Services\",\"item\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/pathology\\\/reference-labs\\\/histology-immunohistochemistry-laboratory\\\/laboratory-services-2\\\/\"},{\"@type\":\"ListItem\",\"position\":5,\"name\":\"Muscle Enzyme Histochemistry\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/pathology\\\/#website\",\"url\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/pathology\\\/\",\"name\":\"Department of Pathology and Laboratory Medicine\",\"description\":\"Department of Pathology and Laboratory Medicine\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/lsom.uthscsa.edu\\\/pathology\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Muscle Enzyme Histochemistry - Department of Pathology and Laboratory Medicine","description":"Muscle biopsy is an essential component of the investigation of patients with neuromuscular disorders (and is also often employed in research studies on the effects of exercise on muscle).","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/histology-immunohistochemistry-laboratory\/laboratory-services-2\/muscle-enzyme-histochemistry\/","og_locale":"en_US","og_type":"article","og_title":"Muscle Enzyme Histochemistry - Department of Pathology and Laboratory Medicine","og_description":"Muscle biopsy is an essential component of the investigation of patients with neuromuscular disorders (and is also often employed in research studies on the effects of exercise on muscle).","og_url":"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/histology-immunohistochemistry-laboratory\/laboratory-services-2\/muscle-enzyme-histochemistry\/","og_site_name":"Department of Pathology and Laboratory Medicine","article_modified_time":"2026-02-02T18:04:13+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/histology-immunohistochemistry-laboratory\/laboratory-services-2\/muscle-enzyme-histochemistry\/","url":"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/histology-immunohistochemistry-laboratory\/laboratory-services-2\/muscle-enzyme-histochemistry\/","name":"Muscle Enzyme Histochemistry - Department of Pathology and Laboratory Medicine","isPartOf":{"@id":"https:\/\/lsom.uthscsa.edu\/pathology\/#website"},"datePublished":"2018-12-11T20:35:34+00:00","dateModified":"2026-02-02T18:04:13+00:00","description":"Muscle biopsy is an essential component of the investigation of patients with neuromuscular disorders (and is also often employed in research studies on the effects of exercise on muscle).","breadcrumb":{"@id":"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/histology-immunohistochemistry-laboratory\/laboratory-services-2\/muscle-enzyme-histochemistry\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/histology-immunohistochemistry-laboratory\/laboratory-services-2\/muscle-enzyme-histochemistry\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/histology-immunohistochemistry-laboratory\/laboratory-services-2\/muscle-enzyme-histochemistry\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/lsom.uthscsa.edu\/pathology\/"},{"@type":"ListItem","position":2,"name":"Laboratories of Department of Pathology and Laboratory Medicine","item":"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/"},{"@type":"ListItem","position":3,"name":"Histology and Immunohistochemistry Laboratory","item":"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/histology-immunohistochemistry-laboratory\/"},{"@type":"ListItem","position":4,"name":"Laboratory Services","item":"https:\/\/lsom.uthscsa.edu\/pathology\/reference-labs\/histology-immunohistochemistry-laboratory\/laboratory-services-2\/"},{"@type":"ListItem","position":5,"name":"Muscle Enzyme Histochemistry"}]},{"@type":"WebSite","@id":"https:\/\/lsom.uthscsa.edu\/pathology\/#website","url":"https:\/\/lsom.uthscsa.edu\/pathology\/","name":"Department of Pathology and Laboratory Medicine","description":"Department of Pathology and Laboratory Medicine","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/lsom.uthscsa.edu\/pathology\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/lsom.uthscsa.edu\/pathology\/wp-json\/wp\/v2\/pages\/836","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lsom.uthscsa.edu\/pathology\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lsom.uthscsa.edu\/pathology\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lsom.uthscsa.edu\/pathology\/wp-json\/wp\/v2\/users\/161"}],"replies":[{"embeddable":true,"href":"https:\/\/lsom.uthscsa.edu\/pathology\/wp-json\/wp\/v2\/comments?post=836"}],"version-history":[{"count":0,"href":"https:\/\/lsom.uthscsa.edu\/pathology\/wp-json\/wp\/v2\/pages\/836\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/lsom.uthscsa.edu\/pathology\/wp-json\/wp\/v2\/pages\/825"}],"wp:attachment":[{"href":"https:\/\/lsom.uthscsa.edu\/pathology\/wp-json\/wp\/v2\/media?parent=836"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}