DepartmentCellular and Integrative Physiology
Martin Paukert, M.D.
Eberhard Karls University of Tübingen, Germany (M.D.)
The Johns Hopkins University School of Medicine (Postdoc)
The Paukert Lab is interested in the role of neuron-astroglia interaction in neuromodulation. Astroglia are recognized for their homeostatic support functions during neuronal activity. Much less is known about how astroglia modulate neuronal activity in a behavioral state-dependent manner. A particular focus of our work lies on understanding molecular events and behavioral context leading to astroglia Ca2+ dynamics in awake mice, consequences for neuronal signaling and alterations of these signals in transgenic mouse models of neurodegenerative and neurobehavioral disease. We are pursuing these goals combining behavioral manipulations, such as employing a motorized treadmill to monitor and control the state of arousal, with two-photon microscopy and electrophysiology to observe activity of ensembles of neurons and astroglia with cellular resolution.
|Noradrenergic terminal short-term potentiation enables modality-selective integration of sensory input and vigilance state. Gray, S., Ye L., Yong Ye, J., and Paukert, M., Science Advances vol 7.51, 2021. DOI: 10.1126/SCIADV.ABK1378|
|Potential and realized impact of astroglia Ca2+ dynamics on circuit function and behavior. Lim, E. Y., Ye, L., Paukert, M., Frontiers in Cellular Neuroscience Jun 7;15:682888, 2021. DOI: 10.3389/fncel.2021.682888|
|Ethanol abolishes vigilance-dependent astroglia network activation in mice by inhibiting norepinephrine release. Ye, L., Orynbayev, M., Zhu, X., Lim, E. Y., Dereddi, R. R., Agarwal, A., Bergles, D. E., Bhat, M. A., Paukert, M., Nature Communications 11(1):6157, 2020. DOI: 10.1038/s41467-020-19475-5 Editors’ Highlights: https://www.nature.com/collections/mjkksldswr/content/christian-schnell|
|Astroglial FMRP deficiency cell-autonomously up-regulates miR-128 and disrupts developmental astroglial mGluR5 signaling. Men, Y., Ye, L., Risgaard, R. D., Promes, V., Zhao, X., Paukert, M., Yang, Y., Proceedings of the National Academy of Sciences USA 117(40):25092-25103, 2020. DOI: 10.1073/pnas.2014080117|
|Reorganization of Destabilized Nodes of Ranvier in β IV Spectrin Mutants Uncovers Critical Timelines for Nodal Restoration and Prevention of Motor Paresis. Saifetiarova, J., Shi, Q., Paukert, M., Komada, M., Bhat, M. A., Journal of Neuroscience 38(28):6267-6282, 2018. DOI: 10.1523/JNEUROSCI.0515-18.2018|
|Comparison of GCaMP3 and GCaMP6f for studying astrocyte Ca2+ dynamics in the awake mouse brain. Ye, L., Haroon, M. A., Salinas, A., Paukert, M., PLoS One 12(7):e0181113, 2017. DOI: 10.1371/journal.pone.0181113|
|Norepinephrine controls astroglial responsiveness to local circuit activity. Paukert, M., Agarwal, A., Jaepyeong, C., Doze, V.A., Kang, J.U. and Bergles, D.E., Neuron 82(6): 1263-1270, 2014. DOI: 10.1016/j.neuron.2014.04.038|
|Reduction of motion artifacts during in vivo two-photon imaging of brain through heartbeat triggered scanning. Paukert, M. and Bergles, D.E., Journal of Physiology 590(Pt 13): 2955-2963, 2012. DOI: 10.1113/jphysiol.2012.228114|
|Zones of enhanced glutamate release from climbing fibers in the mammalian cerebellum. Paukert, M., Huang, Y.H., Tanaka, K., Rothstein, J.D. and Bergles, D.E., Journal of Neuroscience 30: 7290-7299, 2010. DOI: 10.1523/JNEUROSCI.5118-09.2010|