Containers of the appropriate fixatives should be supplied by the hospital histopathology laboratory or if the specimen is to be referred to UT Health San Antonio. They may be requested from the department of pathology histopathology laboratory. These containers are labeled with the proper identification of the fluid contents.
1) One vial of Phosphate Buffered 4% Formaldehyde – 1% Glutaraldehyde Fixative (4CF1G) – Electron Microscopy Fixative
|Solution A||Solution B
|Sodium Phosphate, monobasic - 1.16g||40% Formaldehyde - 10.00 ml|
|Sodium Hydroxide - 0.27g||50% Glutaraldehyde - 2.00 ml|
|Distilled Water - 80.00ml|
2) One vial of Immunofluorescent Transport Medium ( i.e., Zeuss Fixative available commercially from Scientific Products, Immunofix or Buffered Ammonium Sulfate Fixative )
|1 M Potassium Citrate(Citric Acid) Buffer, |
pH 7.0 ( 7.7g / 25 ml D H2O, adjust pH to 7.0 )
|0.1 M Magnesium Sulfate ( 60 mg / 50 ml D H2O )||50 ml|
|0.1 M N-Ethyl Maleimide ( 62 mg / 50 ml D H2O )||50 ml|
|Distilled Water||875 ml|
|Adjust the pH to 7.0 with 1 M Potassium Hydroxide|
( 5.6 g / 100 ml D H2O ) and store refrigerated.
|Buffered Ammonium Sulfate Fixative|
|Ammonium Sulfate, (NH4)2SO4||110 g|
|Buffer Solution, pH 7.0||200 ml
- Patient name and identification on specimen container.
- A completed Patient History and Physical Examination Form.
- A copy of the patient’s Hospital Admission Form.
Preparation of the specimen:
When the specimen is removed from the patient ( ideally, two needle core samples) a portion or one core is placed in the 4CF1G fixative vial ( from which light and electron microscopy evaluation will be made ) and the another portion or one core is placed in the Fluorescent Transport Medium vial. Once the specimen has been placed into the fixatives the two vials may be kept at room temperature.