BCR-ABL P190 Quantitation by Real-Time PCR

CPT Code(s)

81207; G0452-26

Synonym(s)

Hematopathology, CML, ALL

Performed

Molecular Diagnostics Laboratory

Methodology

RNA is isolated, reverse transcribed and amplified by real-time PCR using specific primers
targeting the p190 BCR-ABL and ABL genes. Quantitative results are obtained by comparing
relative levels of p190 BCR-ABL and ABL transcripts to standard curves. Results are reported as
a p190 BCR-ABL to ABL ratio after calibration with a p190 BCR-ABL positive tumor cell line.

Minimum Specimen Requirements

Collect

  • Peripheral blood (PB): 3-5 mL, in purple top (sodium EDTA) tube; yellow top tube
    (ACD) acceptable.
  • Bone marrow (BM): 1-3 mL, drawn into a syringe containing anticoagulant and then
    delivered in a purple top tube.

Transport
Deliver immediately at 2-8°C (wet ice or cold packs). Do not freeze.

Stability
Ambient – 1 hour; refrigerated – 48 hours.
Note: for RNA based assays, samples should be transported to the laboratory within 8 hours of
collection (optimal), or up to a maximum of 48 hours after collection to avoid RNA degradation.
RNA integrity is critical, especially for samples used for monitoring minimal residual disease.

Unacceptable Conditions
Serum or plasma; frozen PB or BM; clotted blood; severely hemolyzed samples.

Turnaround time

Five to seven working days