BCR-ABL P190 Quantitation by Real-Time PCR
Hematopathology, CML, ALL
Molecular Diagnostics Laboratory
RNA is isolated, reverse transcribed and amplified by real-time PCR using specific primers
targeting the p190 BCR-ABL and ABL genes. Quantitative results are obtained by comparing
relative levels of p190 BCR-ABL and ABL transcripts to standard curves. Results are reported as
a p190 BCR-ABL to ABL ratio after calibration with a p190 BCR-ABL positive tumor cell line.
Minimum Specimen Requirements
- Peripheral blood (PB): 3-5 mL, in purple top (sodium EDTA) tube; yellow top tube
- Bone marrow (BM): 1-3 mL, drawn into a syringe containing anticoagulant and then
delivered in a purple top tube.
Deliver immediately at 2-8°C (wet ice or cold packs). Do not freeze.
Ambient – 1 hour; refrigerated – 48 hours.
Note: for RNA based assays, samples should be transported to the laboratory within 8 hours of
collection (optimal), or up to a maximum of 48 hours after collection to avoid RNA degradation.
RNA integrity is critical, especially for samples used for monitoring minimal residual disease.
Serum or plasma; frozen PB or BM; clotted blood; severely hemolyzed samples.
Five to seven working days