BCR::ABL1 p190 Quantitation by Real-Time PCR

CPT Code(s)

81207; G0452

Synonym(s)

Hematopathology, CML, ALL, BCR::ABL1, p190, BCR-ABL1, t(9;22)

Performed

Molecular Diagnostics Laboratory

Clinical Indication and Relevance

The assay can confirm the initial diagnosis of p190 BCR::ABL1 positive acute lymphoblastic leukemia (ALL) or chronic myelogenous leukemia (CML). The assay is also recommended for monitoring minimal residual disease in follow-up samples.

Methodology

RNA is isolated, reverse transcribed and amplified by real-time PCR using specific primers targeting the p190 BCR::ABL1 and ABL1 genes. Quantitative results are obtained by comparing relative levels of p190 BCR::ABL1 and ABL1 transcripts to standard curves. Results are reported as a p190 BCR::ABL1 to ABL1 ratio after calibration with a p190 BCR::ABL1 positive tumor cell line. This assay can detect p190 BCR::ABL1 transcripts to a sensitivity of 1 tumor cell in 1000 normal cells.

Minimum Specimen Requirements

Collect

• Peripheral blood (PB): 3-5 mL, in purple top (sodium EDTA) tube; yellow top tube (ACD) acceptable.
• Bone marrow (BM): 1-3 mL, drawn into a syringe containing anticoagulant and then delivered in a purple top tube.

Transport Deliver immediately at 2-8°C (wet ice or cold packs). Do not freeze.

Stability Ambient – 1 hour; refrigerated – 48 hours. Note: for RNA based assays, samples should be transported to the laboratory within 8 hours of collection (optimal), or up to a maximum of 48 hours after collection to avoid RNA degradation. RNA integrity is critical, especially for samples used for monitoring minimal residual disease.

Unacceptable Conditions Serum or plasma; frozen PB or BM; clotted blood; severely hemolyzed samples.

Turnaround time

Five to seven working days

View Requisition Sheet