Chromosome Mosaicism Analysis (RCA-MOS)

CPT Code(s)

a. Peripheral Blood – 88230, 88263, 88280, 88291 b. Skin – 88233, 88263, 88280, 88291

Synonym(s)

Mosaicism Study

Performed

In-House

Methodology

PHA stimulated short-term culture / In Situ long-term culture initiated using Collagenase for enzyme digestion; chromosome preparation and banding; microscopic analysis of up to 50 cells and karyotype; interpretation and report

Panel/Profile Components

N/A

Critical Values

N/A

Specimen Collection / Handling Requirements

  1. Peripheral blood collected in green top – sodium heparin tube (Peripheral Blood)
  2. Collected in sterile specimen cup or sterile 15 mL tube with RPMI or sterile saline (Skin)
  3. Store and ship at room temperature
  4. Avoid freezing or heating
  5. Ship within 24 hours
  6. Delay > 24 hours – Refrigerate the sample
  7. A complete requisition slip must accompany all specimens and the specimen must be labeled with the patient’s name and identification
  8. ANY SPECIMENS RECEIVED WITHOUT A COMPLETE REQUISTION OR AN UNLABELED SPECIMEN WILL BE REJECTED.
  9. All requisition must have a valid ICD-10 code/clinical indication and must have the name of an authorized Any sample without this information will be rejected.

Minimum Specimen Requirements

Optimal Quantity: 4-5 ml; Minimum Quantity: 1-2 ml (Peripheral Blood); Optimal Quantity: 2 cm3; Minimum Quantity: 1 cm3 (Skin)

Turnaround time

Total testing time: 10 days (Peripheral Blood), 6 weeks (Skin)

Communication

  1. Turnaround time non-conformity: Email or call Pathologist on request form to notify them of the
  2. Technical Updates: Email Applicable Laboratory

Quality

QA/Utilization Report