Fluorescence In Situ Hybridization for Microdeletions (FISH-DEL)
CPT Code(s)
88271x2, 88273, 88291
Synonym(s)
FISH for Prader-Willi Syndrome / FISH for 22q11.2 microdeletion etc.
Performed
In-House
Methodology
PHA stimulated short-term culture; metaphase cell slide preparation; DNA denaturation with target probe and hybridization followed by post-hybridization wash; DAPI application for fluorescence microscopic analysis of chromosomes; interpretation and report (Peripheral Blood)
In Situ long-term culture; chromosome preparation; DNA denaturation with target probe and hybridization followed by post-hybridization wash; DAPI application for fluorescence microscopic analysis of chromosomes; interpretation and report (Amniotic Fluid / Products of Conception / Skin)
Panel/Profile Components
N/A
Critical Values
N/A
Specimen Collection / Handling Requirements
- Peripheral blood collected in green top – sodium heparin tube (Peripheral Blood)
- Collected in sterile specimen cup or sterile 15 mL tube with RPMI or sterile saline (Amniotic Fluid / Products of Conception / Skin)
- Store and ship at room temperature
- Avoid freezing or heating
- Ship within 24 hours
- Delay > 24 hours – Refrigerate the sample
- A complete requisition slip must accompany all specimens and the specimen must be labeled with the patient’s name and identification
- ANY SPECIMENS RECEIVED WITHOUT A COMPLETE REQUISTION OR AN UNLABELED SPECIMEN WILL BE REJECTED.
- All requisition must have a valid ICD-10 code/clinical indication and must have the name of an authorized Any sample without this information will be rejected.
Minimum Specimen Requirements
Optimal Quantity: 4-5 ml; Minimum Quantity: 1-2 ml (Peripheral Blood) ,Optimal Quantity: 2 cm3; Minimum Quantity: 1 cm3 (Skin)
Turnaround time
Total testing time: 7 days
Communication
- Turnaround time non-conformity: Email or call Pathologist on request form to notify them of the
- Technical Updates: Email Applicable Laboratory
Quality
QA/Utilization Report