Fluorescence In Situ Hybridization on Amniotic Fluid (FISH-ANU)

CPT Code(s)

88271x3, 88273, 88291

Synonym(s)

Rapid FISH / Rapid Prenatal FISH / Rapid FISH Screen for Aneuploidies

Performed

In-House

Methodology

Direct harvest of amniocytes; interphase cell slide preparation; DNA denaturation with target probe and hybridization followed by post-hybridization wash; DAPI application for fluorescence microscopic analysis of nuclei; interpretation and report

Panel/Profile Components

N/A

Critical Values

N/A

Specimen Collection / Handling Requirements

  1. Amniotic fluid collected in sterile 15 mL tubes (discard first 1 cc of amniotic fluid)
  2. Store and ship at room temperature
  3. Avoid freezing or heating
  4. Ship within 24 hours
  5. Delay > 24 hours – Refrigerate the sample
  6. A complete requisition slip must accompany all specimens and the specimen must be labeled with the patient’s name and identification
  7. ANY SPECIMENS RECEIVED WITHOUT A COMPLETE REQUISTION OR AN UNLABELED SPECIMEN WILL BE REJECTED.
  8. All requisition must have a valid ICD-10 code/clinical indication and must have the name of an authorized Any sample without this information will be rejected.

Minimum Specimen Requirements

Optimal Quantity: > 15 ml; Minimum Quantity: 5 ml

Turnaround time

Total testing time: 7 days

Communication

  1. Turnaround time non-conformity: Email or call Pathologist on request form to notify them of the
  2. Technical Updates: Email Applicable Laboratory

Quality

QA/Utilization Report