Fluorescence In Situ Hybridization on Amniotic Fluid (FISH-ANU)
CPT Code(s)
88271x3, 88273, 88291
Synonym(s)
Rapid FISH / Rapid Prenatal FISH / Rapid FISH Screen for Aneuploidies
Performed
In-House
Methodology
Direct harvest of amniocytes; interphase cell slide preparation; DNA denaturation with target probe and hybridization followed by post-hybridization wash; DAPI application for fluorescence microscopic analysis of nuclei; interpretation and report
Panel/Profile Components
N/A
Critical Values
N/A
Specimen Collection / Handling Requirements
- Amniotic fluid collected in sterile 15 mL tubes (discard first 1 cc of amniotic fluid)
- Store and ship at room temperature
- Avoid freezing or heating
- Ship within 24 hours
- Delay > 24 hours – Refrigerate the sample
- A complete requisition slip must accompany all specimens and the specimen must be labeled with the patient’s name and identification
- ANY SPECIMENS RECEIVED WITHOUT A COMPLETE REQUISTION OR AN UNLABELED SPECIMEN WILL BE REJECTED.
- All requisition must have a valid ICD-10 code/clinical indication and must have the name of an authorized Any sample without this information will be rejected.
Minimum Specimen Requirements
Optimal Quantity: > 15 ml; Minimum Quantity: 5 ml
Turnaround time
Total testing time: 7 days
Communication
- Turnaround time non-conformity: Email or call Pathologist on request form to notify them of the
- Technical Updates: Email Applicable Laboratory
Quality
QA/Utilization Report