Human papillomavirus (HPV) by Transcription Mediated Amplification

CPT Code(s)

87624 Human Papillomavirus High-Risk Types

Synonym(s)

HPV, high-risk HPV

Performed

Molecular Diagnostics Laboratory

Clinical Indication and Relevance

The assay is useful to detect 14 high-risk types of Human papillomavirus from cervical specimens collected in ThinPrep Pap Test vials and transferred into the Aptima Specimen transfer tube.

Methodology

The assay uses transcription mediated amplification (TMA) on the Hologic Panther System.
“Minimum Specimen Requirements”

Specimen Collection / Handling Requirements

Sample Type: 

Aptima Specimen Transport Tube

Specimen Labeling:

The specimen must be labeled with two identifiers at the time of collection. Examples of acceptable identifiers include but are not limited to patient name, date of birth, hospital medical record number, accession number. A location (e.g., hospital room number) is not an acceptable identifier. Collection date and collector’s identifier are required.

A completed requisition form should be submitted with every sample, and at minimum, the following is required: ordering physician name, phone number, fax number, and patient’s name, identifying number of patients, patient sex, patient date of birth specimen type, collection date, tests requested, provisional diagnosis or clinical rationale for test, and billing information.

Minimum Specimen Requirements

Transport:

Specimens can contain high levels of organisms.  Specimens must be shipped and transported in accordance with applicable local, national, and international transportation regulations.  Maintain proper storage conditions of 2-8°C during shipping to ensure integrity of the specimen.

Stability:

Aptima Transport tubes:  After collection, transport and store the transport tube at 2-30°C up to 60 days after collection.

Unacceptable Samples: 

Improperly labeled or incorrect patient identification, specimen leaked, or incorrect media. ThinPrep liquid cytology specimens containing less than 1 mL after ThinPrep Pap Test slide preparation are considered inadequate for the Aptima HPV assay.

Turnaround time

1-3 Business Days.

View Requisition Sheet