Immunohistochemistry is a technique for localizing and visualizing an antigen in a tissue section by using an antibody specific for the target antigen. The immunohistochemistry procedure consists of tissue preparation, antibody incubation, and a series of detection reactions. Technically, when the procedure is performed on cells (cell smears, cytocentrifuge preps, etc.) rather than tissue sections, it is termed immunocytochemistry, but in practice the two terms are often used interchangeably.

AntibodyCommon ApplicationsCPT
Alpha-1Antichymotrypsin, polyclonal, IHCHistocytic Marker88342
Alpha-Synueclein, polyclonal, IHCAlzheimer Disease88342
ACTH (Adreno Corticotrophic Hormone), polyclonal, IHCPituitary Tumors88342
Actin Muscle, smooth, monoclonal, IHCSmooth Muscle88342
AFP (Alpha-Fetoprotein), polyclonal, IHCHepatoma, Germ Cell Tumor88342
ALK-1, monoclonal, IHCALK-1 Positive Lymphoma88342
Ber EP4, monoclonal, IHCMesothelioma vs Adenocarcinoma88342
B 72.3, monoclonal, IHCMesothelioma vs Adenocarcinoma88342
Bcl-2, monoclonal, IHCLymphoma Typing, Soft Tissue Tumors88342
Bcl-6, monoclonal, IHCLymphoma Typing88342
BOB-1, polyclonal, IHCB-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, and nodular lymphocyte predominant Hodgkin lymphoma.88342
CA19-9, monoclonal, IHCMetastatic Carcinoma Unknown Primary88342
CA125, monoclonal, IHCMesothelioma vs. Adenocarcinoma88342
CAM5.2, monoclonal, IHCAdenocarcinoma88342
Calretinin, monoclonal, IHCMedullary carcinoma thyroid88342
Calcitonin, polyclonal, IHCMedullary carcinoma thyroid88342
Calponin, monoclonal, IHCSmooth Muscle differentiation, myoepithelial cells88342
Carbonic anhydrase IX, CA9Carbonic anhydrase IX; renal cell carcinoma (RCC); distinguish clear cell renal cell carcinoma from other RCC subset88342
C-Kit, CD117, monoclonal, IHCGastrointestinal stromal tumors mast cells88342
c-erbB-2, Her 2, monoclonal, IHCBreast Cancer 88360
CEA (Carcinoembryonic Antigen),monoclonal, IHCAdenocarcinoma, metastatic carcinoma unknown primary88342
CD1a, monoclonal, IHCThymic T-cells, thymoma, langerhans cells88342
CD2, monoclonal, IHCLymphoma/leukemia typing88342
CD3, monoclonal, IHCLymphoma/leukemia typing88342
CD4, monoclonal, IHCLymphoma/leukemia typing88342
CD5, monoclonal, IHCThymic carcinoma88342
CD7, monoclonal, IHCLymphoma/leukemia typing88342
CD8, monoclonal, IHCLymphoma/leukemia typing88342
CD10, monoclonal, IHCLymphoma typing, metastatic carcinoma unknown primary88342
CD15, monoclonal, IHCLymphoma typing, mesothelioma vs. adenocarcinoma88342
CD20, monoclonal, IHCLymphoma/leukemia typing88342
CD21, monoclonal, IHCFollicular dendritic cells, lymphoma typing88342
CD23, monoclonal, IHCLymphoma/leukemia typing88342
CD25, monoclonal, IHCSystemic Mastocytosis88342
CD30, monoclonal, IHCLymphoma typing88342
CD31, monoclonal, IHCVascular differentiation88342
CD33,monoclonal, IHCIdentification of cells of myeloid and monocytic lineage, and leukemias and myeloproliferative disorders derived from these cells.88342
CD34, monoclonal, IHCSoft tissue tumor classification, leukemia typing88342
CD43, monoclonal, IHCLymphoma/leukemia typing88342
D45RB, monoclonal, IHCRecognition of lymphohematopoetic tumors88342
CD45RO, monoclonal, IHCLymphoma typing (same as UCHL-1)88342
CD56, monoclonal, IHCLymphoma typing, neuroendocrine differentiation88342
CD57, monoclonal, IHCLymphoma typing, neuroendocrine differentiation88342
CD61, monoclonal, IHCMicromegakaryocytes in myelodysplastic syndrome, acute myeloid leukemia with multilineage dysplasia, acute megakaryoblastic leukemia, and myeloproliferative neoplasms88342
CD68, monoclonal, IHCHistocytic/ myeloid/monocytic marker88342
CD71,monoclonal, IHErythroid leukemia, benign erythroid proliferative disorders, and myelodysplastic syndrome88342
CD79a, monoclonal, IHCLymphoma/leukemia typing88342
CD99, monoclonal, IHCSoft tissue tumor classification88342
CD123, monoclonal, IHCLymphoma/leukemia typing88342
CD138, monoclonal, IHCPlasma Cells (also stains many epithelial tumors)88342
CD 207, Langerin, monoclonal, IHCDetect Langerin in cells and tissues88342
CDK4, monoclonal, IHCLiposarcoma88342
Chromogranin, monoclonal, IHCNeuroendocrine differentiation88342
CLA (Cutaneous Lymphocyte Antigen, Rat, IHCCutaneous Lymphocytes88342
Clusterin, monoclonal, IHCAnaplastic Large Cell Lymphoma88342
CMV (Cytomegalovirus), monoclonal, IHCCytomegalovirus infection88342
C-MYC, rabbit monoclonal, IHCBurkitt lymphoma88342
CXCL-13, monoclonal, IHCAngioimmunoblastic T-cell lymphoma88342
Cyclin D1, monoclonal, IHCMantle cell lymphoma88342
Cytokeratin 5/6, monoclonal, IHCDistinguish lung squamous cell carcinoma from lung adenocarcinoma and large cell carcinoma88342
Cytokeratin 7, monoclonal, IHCMetastatic carcinoma unknown primary88342
Cytokeratin 20, monoclonal, IHCMetastatic carcinoma unknown primary88342
D2-40, monoclonal, IHCLymphatic invasion of a variety of cancers88342
Desmin, monoclonal, IHCSmooth and skeletal muscle differentiation88342
EBER, ISHEpstein Bar Virus88368
E-Cadherin, monoclonal, IHCLobular vs. Ductal breast carcinoma88342
EGFR, monoclonal, IHCSweat gland carcinoma, squamous tumors88342
EMA (Epithelial Membrane Antigen), monoclonal, IHCMetastatic carcinoma of unknown primary, lymphoma typing88342
Epstein Barr Virus (LMP) , monoclonal, IHCEpstein Bar Virus88342
Estrogen Receptor, rabbit monoclonal, IHCBreast cancer prognostic marker, metastatic carcinoma unknown primary88360
Factor VIII-Related Antigen, polyclonal, IHCMegakaryocytic marker, endothelial marker88342
Factor XIII-A-subunit, polyclonal, IHCDermatofibroma vs. DFSP88342
Fascin, monoclonal, IHCHodgkin's Lymphoma88342
FSH (Follicle Stimulating Hormone), polyclonal, IHCPituitary tumors88342
Gastrin, polyclonal, IHCGastrinomas88342
GCDFP-15(Gross Cystic Disease Fluid Protein-15), monoclonal, IHCBreast Cancer88342
GFAP (Glial Fibrillary Acid Protein), monoclonal, IHCGlial tumors, salivary gland tumors88342
GH (Growth Hormone), polyclonal, IHCPituitary tumors88342
Glucagon, polyclonal, IHCPancreatic endocrine tumors88342
Glycophorin A, monoclonal, IHCCharacterize erythroid cell development and in the diagnosis of erythroid leukemia88342
Glypican-3 (GPC-3), monoclonal, IHCHepatocellular carcinoma (HCC)88342
HBME-1, monoclonal, IHCClassification of mesotheliomas88342
HCG-Beta (Human Chorionic Gonadotropin), polyclonal, IHCChoriocarcinoma88342
Helicobacter Pylori, polyclonal, IHCHelicobacter Pylori88342
Hemoglobin A, polyclonal, IHCErythroid differentiation in various myeloproliferative and dysmyelopoietic syndromes88342
Hepar, monoclonal, IHCHepacellular carcinoma88342
Herpes Simplex Virus Type I, polyclonal, IHCHerpesvirus I and II infection88342
Herpes Simplex Virus Type II, polyclonal, IHCHerpesvirus I and II infection88342
HHV-8, IHCKaposi sarcoma88342
HMB45 (Melanoma) , monoclonal, IHCMelanoma marker, angiomyolipoma88342
IgA (Alpha Chains & Secretary Component),polyclonal, IHCPlasma cell subclassification88342
IgG (Gamma Chains), polyclonal, IHCPlasma cell subclassification88342
IgG4, monoclonal, IHCIgG4 systemic sclerosing disease88342
IgM (Mu Chains), polyclonal, IHCPlasma cell subclassification88342
IgD, polyclonal, IHCPlasma cell subclassification88342
Inhibin-A, monoclonal, IHCDifferential diagnosis of intra- uterine vs. ectopic pregnancy in endometrial curettings88342
Insulin, monoclonal (GP) , IHCPancreatic endocrine tumors88342
Keratin 903, monoclonal, IHCProstate marker88342
Kappa Light Chains, polyclonal, IHCPlasma cell clonality88342
Ki67, monoclonal, IHCProliferative fraction, fat cells88342
Lambda Light Chains, polyclonal, IHCPlasma cell clonality88342
Laminin, monoclonal IHCSkeletal muscle88342
LH (Lutenizing Hormone), polyclonal, IHCPituitary tumors88342
Lysozyme (Muramidase), polyclonal, IHCMyeloid and histiocytic marker88342
Mammoglobin, monoclonal, IHCBreast cancer88342
MART-1, monoclonal, IHCMelanoma marker, angiomyolipoma88342
Mast Cell Tryptase, monoclonal IHC
Mast Cell Tumors
MDM2, monoclonal, IHC
Liposarcoma or Breast cancer
Myogenin, monoclonal, IHC
Myosin, monoclonal, IHC
Skeletal muscle
MUM-1, monoclonal, IHC
Lymphoma marker
Myeloperoxidase, polyclonal, IHC
Acute leukemia
Napsin A, polyclonal, IHC
Lung adenocarcinoma
Neurofilament, monoclonal, IHCNeural tumors
NSE (Neuron Specific Enolase), monoclonal, IHC
Pancreatic endocrine tumors
OCT-2, polyclonal, IHC
Lymphoma marker
OCT 3/4, monoclonal, IHC
Germ cell marker
P16, monoclonal, IHC
Cervical dysplasia (correlates with high risk HPV)
P21, monoclonal, IHC
Gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma
p53,(DO7), monoclonal, IHC
Breast Carcinoma
P63, monoclonal, IHC
Prostatic basal cells- Prostate marker
Pan-Actin Muscle (HHF35), monoclonal, IHC
Smooth and Skeletal Muscle
Pan Keratin( AE1/AE3+ PCK26),monoclonal, IHC
Epithelial tumors
Pan-Melanoma Cocktail (HMB-45, Mart-1, Tyrosianase), monoclonal, IHC
Parathyroid Hormone (PTH), polyclonal, IHC
Parathyroid tumors
Pax 5, monoclonal, IHC
B-Cell Lymphoma
PAX-8Renal cell carcinoma88342
PD1, monoclonal, IHC
Follicular Helper T-Cells
PIN-4 Cocktail, (CK5+CK14+p63+P504S), IHC
Prostate marker
Placental Alkaline Phosphatase, polyclonal, IHC
Germ Cell Tumor
Polyoma Virus, monoclonal, IHC
Polyoma Virus – SV-40
Progesterone Receptor, rabbit monoclonal, IHC
Breast prognostic marker
Prolactin, polyclonal, IHC
Pituitary tumors
PSA (Prostate Specific Antigen), monoclonal, IHC
Prostate marker
PSMA, polyclonal, IHC
Prostate marker
S100, monoclonal, IHC
Spitz nevus
Seratonin, monoclonal, IHC
Somatostatin, polyclonal, IHC
Pancreatic endocrine tumors
Sox-11, polyclonal, IHC
Blastoid Mantle Cell Lymphoma
Spirochete ,polyclonal, IHC
Synaptophysin, monoclonal, IHC
Neuroendocrine differentiation
TCL-1A, monoclonal, IHC
TCRbf1,monoclonal, IHC
Lymphoma typing
TCR Gamma 3.2, monoclonal, IHC
Characterize T-cells in lymphomas and inflammation
TIA-1, monoclonal, IHC
Lymphoma typing
TDT, polyclonal, IHC
Lymphoma/leukemia typing
TTF-1, monoclonal, IHC
Lung & thyroid marker, also some neuroendocrine
Thyroglobulin, monoclonal, IHC
Thyroid carcinoma
TSH (Thyroid Stimulating Hormone), polyclonal, IHC
Pituitary tumors
Vimentin, monoclonal, IHC
Metastatic Carcinoma Unknown Primary