Immunohistochemistry
Immunohistochemistry is a technique for localizing and visualizing an antigen in a tissue section by using an antibody specific for the target antigen. The immunohistochemistry procedure consists of tissue preparation, antibody incubation, and a series of detection reactions. Technically, when the procedure is performed on cells (cell smears, cytocentrifuge preps, etc.) rather than tissue sections, it is termed immunocytochemistry, but in practice the two terms are often used interchangeably.
| Antibody | Common Applications | CPT |
|---|---|---|
| Alpha-1Antichymotrypsin, polyclonal, IHC | Histocytic Marker | 88342 |
| Alpha-Synueclein, polyclonal, IHC | Alzheimer Disease | 88342 |
| ACTH (Adreno Corticotrophic Hormone), polyclonal, IHC | Pituitary Tumors | 88342 |
| Actin Muscle, smooth, monoclonal, IHC | Smooth Muscle | 88342 |
| AFP (Alpha-Fetoprotein), polyclonal, IHC | Hepatoma, Germ Cell Tumor | 88342 |
| ALK-1, monoclonal, IHC | ALK-1 Positive Lymphoma | 88342 |
| Ber EP4, monoclonal, IHC | Mesothelioma vs Adenocarcinoma | 88342 |
| B 72.3, monoclonal, IHC | Mesothelioma vs Adenocarcinoma | 88342 |
| Bcl-2, monoclonal, IHC | Lymphoma Typing, Soft Tissue Tumors | 88342 |
| Bcl-6, monoclonal, IHC | Lymphoma Typing | 88342 |
| BOB-1, polyclonal, IHC | B-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, and nodular lymphocyte predominant Hodgkin lymphoma. | 88342 |
| CA19-9, monoclonal, IHC | Metastatic Carcinoma Unknown Primary | 88342 |
| CA125, monoclonal, IHC | Mesothelioma vs. Adenocarcinoma | 88342 |
| CAM5.2, monoclonal, IHC | Adenocarcinoma | 88342 |
| Calretinin, monoclonal, IHC | Medullary carcinoma thyroid | 88342 |
| Calcitonin, polyclonal, IHC | Medullary carcinoma thyroid | 88342 |
| Calponin, monoclonal, IHC | Smooth Muscle differentiation, myoepithelial cells | 88342 |
| Carbonic anhydrase IX, CA9 | Carbonic anhydrase IX; renal cell carcinoma (RCC); distinguish clear cell renal cell carcinoma from other RCC subset | 88342 |
| C-Kit, CD117, monoclonal, IHC | Gastrointestinal stromal tumors mast cells | 88342 |
| c-erbB-2, Her 2, monoclonal, IHC | Breast Cancer | 88360 |
| CEA (Carcinoembryonic Antigen),monoclonal, IHC | Adenocarcinoma, metastatic carcinoma unknown primary | 88342 |
| CD1a, monoclonal, IHC | Thymic T-cells, thymoma, langerhans cells | 88342 |
| CD2, monoclonal, IHC | Lymphoma/leukemia typing | 88342 |
| CD3, monoclonal, IHC | Lymphoma/leukemia typing | 88342 |
| CD4, monoclonal, IHC | Lymphoma/leukemia typing | 88342 |
| CD5, monoclonal, IHC | Thymic carcinoma | 88342 |
| CD7, monoclonal, IHC | Lymphoma/leukemia typing | 88342 |
| CD8, monoclonal, IHC | Lymphoma/leukemia typing | 88342 |
| CD10, monoclonal, IHC | Lymphoma typing, metastatic carcinoma unknown primary | 88342 |
| CD15, monoclonal, IHC | Lymphoma typing, mesothelioma vs. adenocarcinoma | 88342 |
| CD20, monoclonal, IHC | Lymphoma/leukemia typing | 88342 |
| CD21, monoclonal, IHC | Follicular dendritic cells, lymphoma typing | 88342 |
| CD23, monoclonal, IHC | Lymphoma/leukemia typing | 88342 |
| CD25, monoclonal, IHC | Systemic Mastocytosis | 88342 |
| CD30, monoclonal, IHC | Lymphoma typing | 88342 |
| CD31, monoclonal, IHC | Vascular differentiation | 88342 |
| CD33,monoclonal, IHC | Identification of cells of myeloid and monocytic lineage, and leukemias and myeloproliferative disorders derived from these cells. | 88342 |
| CD34, monoclonal, IHC | Soft tissue tumor classification, leukemia typing | 88342 |
| CD43, monoclonal, IHC | Lymphoma/leukemia typing | 88342 |
| D45RB, monoclonal, IHC | Recognition of lymphohematopoetic tumors | 88342 |
| CD45RO, monoclonal, IHC | Lymphoma typing (same as UCHL-1) | 88342 |
| CD56, monoclonal, IHC | Lymphoma typing, neuroendocrine differentiation | 88342 |
| CD57, monoclonal, IHC | Lymphoma typing, neuroendocrine differentiation | 88342 |
| CD61, monoclonal, IHC | Micromegakaryocytes in myelodysplastic syndrome, acute myeloid leukemia with multilineage dysplasia, acute megakaryoblastic leukemia, and myeloproliferative neoplasms | 88342 |
| CD68, monoclonal, IHC | Histocytic/ myeloid/monocytic marker | 88342 |
| CD71,monoclonal, IH | Erythroid leukemia, benign erythroid proliferative disorders, and myelodysplastic syndrome | 88342 |
| CD79a, monoclonal, IHC | Lymphoma/leukemia typing | 88342 |
| CD99, monoclonal, IHC | Soft tissue tumor classification | 88342 |
| CD123, monoclonal, IHC | Lymphoma/leukemia typing | 88342 |
| CD138, monoclonal, IHC | Plasma Cells (also stains many epithelial tumors) | 88342 |
| CD 207, Langerin, monoclonal, IHC | Detect Langerin in cells and tissues | 88342 |
| CDK4, monoclonal, IHC | Liposarcoma | 88342 |
| Chromogranin, monoclonal, IHC | Neuroendocrine differentiation | 88342 |
| CLA (Cutaneous Lymphocyte Antigen, Rat, IHC | Cutaneous Lymphocytes | 88342 |
| Clusterin, monoclonal, IHC | Anaplastic Large Cell Lymphoma | 88342 |
| CMV (Cytomegalovirus), monoclonal, IHC | Cytomegalovirus infection | 88342 |
| C-MYC, rabbit monoclonal, IHC | Burkitt lymphoma | 88342 |
| CXCL-13, monoclonal, IHC | Angioimmunoblastic T-cell lymphoma | 88342 |
| Cyclin D1, monoclonal, IHC | Mantle cell lymphoma | 88342 |
| Cytokeratin 5/6, monoclonal, IHC | Distinguish lung squamous cell carcinoma from lung adenocarcinoma and large cell carcinoma | 88342 |
| Cytokeratin 7, monoclonal, IHC | Metastatic carcinoma unknown primary | 88342 |
| Cytokeratin 20, monoclonal, IHC | Metastatic carcinoma unknown primary | 88342 |
| D2-40, monoclonal, IHC | Lymphatic invasion of a variety of cancers | 88342 |
| Desmin, monoclonal, IHC | Smooth and skeletal muscle differentiation | 88342 |
| EBER, ISH | Epstein Bar Virus | 88368 |
| E-Cadherin, monoclonal, IHC | Lobular vs. Ductal breast carcinoma | 88342 |
| EGFR, monoclonal, IHC | Sweat gland carcinoma, squamous tumors | 88342 |
| EMA (Epithelial Membrane Antigen), monoclonal, IHC | Metastatic carcinoma of unknown primary, lymphoma typing | 88342 |
| Epstein Barr Virus (LMP) , monoclonal, IHC | Epstein Bar Virus | 88342 |
| Estrogen Receptor, rabbit monoclonal, IHC | Breast cancer prognostic marker, metastatic carcinoma unknown primary | 88360 |
| Factor VIII-Related Antigen, polyclonal, IHC | Megakaryocytic marker, endothelial marker | 88342 |
| Factor XIII-A-subunit, polyclonal, IHC | Dermatofibroma vs. DFSP | 88342 |
| Fascin, monoclonal, IHC | Hodgkin's Lymphoma | 88342 |
| FSH (Follicle Stimulating Hormone), polyclonal, IHC | Pituitary tumors | 88342 |
| Gastrin, polyclonal, IHC | Gastrinomas | 88342 |
| GCDFP-15(Gross Cystic Disease Fluid Protein-15), monoclonal, IHC | Breast Cancer | 88342 |
| GFAP (Glial Fibrillary Acid Protein), monoclonal, IHC | Glial tumors, salivary gland tumors | 88342 |
| GH (Growth Hormone), polyclonal, IHC | Pituitary tumors | 88342 |
| Glucagon, polyclonal, IHC | Pancreatic endocrine tumors | 88342 |
| Glycophorin A, monoclonal, IHC | Characterize erythroid cell development and in the diagnosis of erythroid leukemia | 88342 |
| Glypican-3 (GPC-3), monoclonal, IHC | Hepatocellular carcinoma (HCC) | 88342 |
| HBME-1, monoclonal, IHC | Classification of mesotheliomas | 88342 |
| HCG-Beta (Human Chorionic Gonadotropin), polyclonal, IHC | Choriocarcinoma | 88342 |
| Helicobacter Pylori, polyclonal, IHC | Helicobacter Pylori | 88342 |
| Hemoglobin A, polyclonal, IHC | Erythroid differentiation in various myeloproliferative and dysmyelopoietic syndromes | 88342 |
| Hepar, monoclonal, IHC | Hepacellular carcinoma | 88342 |
| Herpes Simplex Virus Type I, polyclonal, IHC | Herpesvirus I and II infection | 88342 |
| Herpes Simplex Virus Type II, polyclonal, IHC | Herpesvirus I and II infection | 88342 |
| HHV-8, IHC | Kaposi sarcoma | 88342 |
| HMB45 (Melanoma) , monoclonal, IHC | Melanoma marker, angiomyolipoma | 88342 |
| IgA (Alpha Chains & Secretary Component),polyclonal, IHC | Plasma cell subclassification | 88342 |
| IgG (Gamma Chains), polyclonal, IHC | Plasma cell subclassification | 88342 |
| IgG4, monoclonal, IHC | IgG4 systemic sclerosing disease | 88342 |
| IgM (Mu Chains), polyclonal, IHC | Plasma cell subclassification | 88342 |
| IgD, polyclonal, IHC | Plasma cell subclassification | 88342 |
| Inhibin-A, monoclonal, IHC | Differential diagnosis of intra- uterine vs. ectopic pregnancy in endometrial curettings | 88342 |
| Insulin, monoclonal (GP) , IHC | Pancreatic endocrine tumors | 88342 |
| Keratin 903, monoclonal, IHC | Prostate marker | 88342 |
| Kappa Light Chains, polyclonal, IHC | Plasma cell clonality | 88342 |
| Ki67, monoclonal, IHC | Proliferative fraction, fat cells | 88342 |
| Lambda Light Chains, polyclonal, IHC | Plasma cell clonality | 88342 |
| Laminin, monoclonal IHC | Skeletal muscle | 88342 |
| LH (Lutenizing Hormone), polyclonal, IHC | Pituitary tumors | 88342 |
| Lysozyme (Muramidase), polyclonal, IHC | Myeloid and histiocytic marker | 88342 |
| Mammoglobin, monoclonal, IHC | Breast cancer | 88342 |
| MART-1, monoclonal, IHC | Melanoma marker, angiomyolipoma | 88342 |
| Mast Cell Tryptase, monoclonal IHC | Mast Cell Tumors | 88342 |
| MDM2, monoclonal, IHC | Liposarcoma or Breast cancer | 88342 |
| Myogenin, monoclonal, IHC | Rhabdomyosarcoma | 88342 |
| Myosin, monoclonal, IHC | Skeletal muscle | 88342 |
| MUM-1, monoclonal, IHC | Lymphoma marker | 88342 |
| Myeloperoxidase, polyclonal, IHC | Acute leukemia | 88342 |
| Napsin A, polyclonal, IHC | Lung adenocarcinoma | 88342 |
| Neurofilament, monoclonal, IHC | Neural tumors | 88342 |
| NSE (Neuron Specific Enolase), monoclonal, IHC | Pancreatic endocrine tumors | 88342 |
| OCT-2, polyclonal, IHC | Lymphoma marker | 88342 |
| OCT 3/4, monoclonal, IHC | Germ cell marker | 88342 |
| P16, monoclonal, IHC | Cervical dysplasia (correlates with high risk HPV) | 88342 |
| P21, monoclonal, IHC | Gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma | 88342 |
| p53,(DO7), monoclonal, IHC | Breast Carcinoma | 88342 |
| P63, monoclonal, IHC | Prostatic basal cells- Prostate marker | 88342 |
| Pan-Actin Muscle (HHF35), monoclonal, IHC | Smooth and Skeletal Muscle | 88342 |
| Pan Keratin( AE1/AE3+ PCK26),monoclonal, IHC | Epithelial tumors | 88342 |
| Pan-Melanoma Cocktail (HMB-45, Mart-1, Tyrosianase), monoclonal, IHC | Melanoma | 88342 |
| Parathyroid Hormone (PTH), polyclonal, IHC | Parathyroid tumors | 88342 |
| Pax 5, monoclonal, IHC | B-Cell Lymphoma | 88342 |
| PAX-8 | Renal cell carcinoma | 88342 |
| PD1, monoclonal, IHC | Follicular Helper T-Cells | 88342 |
| PIN-4 Cocktail, (CK5+CK14+p63+P504S), IHC | Prostate marker | 88342 |
| Placental Alkaline Phosphatase, polyclonal, IHC | Germ Cell Tumor | 88342 |
| Polyoma Virus, monoclonal, IHC | Polyoma Virus – SV-40 | 88342 |
| Progesterone Receptor, rabbit monoclonal, IHC | Breast prognostic marker | 88360 |
| Prolactin, polyclonal, IHC | Pituitary tumors | 88342 |
| PSA (Prostate Specific Antigen), monoclonal, IHC | Prostate marker | 88342 |
| PSMA, polyclonal, IHC | Prostate marker | 88342 |
| S100, monoclonal, IHC | Spitz nevus | 88342 |
| Seratonin, monoclonal, IHC | Carcinoids | 88342 |
| Somatostatin, polyclonal, IHC | Pancreatic endocrine tumors | 88342 |
| Sox-11, polyclonal, IHC | Blastoid Mantle Cell Lymphoma | 88342 |
| Spirochete ,polyclonal, IHC | Spirochete | 88342 |
| Synaptophysin, monoclonal, IHC | Neuroendocrine differentiation | 88342 |
| TCL-1A, monoclonal, IHC | Leukemia | 88342 |
| TCRbf1,monoclonal, IHC | Lymphoma typing | 88342 |
| TCR Gamma 3.2, monoclonal, IHC | Characterize T-cells in lymphomas and inflammation | 88342 |
| TIA-1, monoclonal, IHC | Lymphoma typing | 88342 |
| TDT, polyclonal, IHC | Lymphoma/leukemia typing | 88342 |
| TTF-1, monoclonal, IHC | Lung & thyroid marker, also some neuroendocrine | 88342 |
| Thyroglobulin, monoclonal, IHC | Thyroid carcinoma | 88342 |
| TSH (Thyroid Stimulating Hormone), polyclonal, IHC | Pituitary tumors | 88342 |
| Vimentin, monoclonal, IHC | Metastatic Carcinoma Unknown Primary | 88342 |