Technical Resources

Technical Resources – Sample Preparation

Preparation of Minimal Salts Media
Growth Procedure for H₂O Minimal Salts Media
Growth Procedure for D₂O Minimal Salts Media
Buffer Exchange Procedure for the Preparation of NMR Samples
Effect of pH on the NMR Spectra

Technical Resources – Operating the NMR Spectrometers & Data Acquisition and Processing

Step-by-step procedure for NMR data acquisition
Chemical shift referencing and temperature calibration
How to process a 2D HSQC spectrum
How to measure and analyze ¹⁵N T2 relaxation times.
Assigning Proteins Using Sparky
NMRPipe Reference Page

Bruker Manuals

Pulse program manual
Processing manual
Acquisition manual